ABSTRACT
The tissue distribution of estrogen receptor alpha (ERα) and progesterone receptor (PR) was examined using immunohistochemical technique. Image analysis was done to quantify the immune reactivity. The ERα and PR was localized in luminal epithelium, glandular epithelium, stromal cells, endothelial cells and myometrium and few cells in perimetrium. The immuno staining was observed in the nuclei of cells, however, faint cytoplasmic staining for PR was also observed. Variations were seen in the different tissue compartments of the uterus and during the different phases of the estrous cycle. Significantly higher number of ERα positive cells was observed in lamina epithelialis as compared to stromal cells and smooth muscle cells in myometrium. Significantly higher percentage of ERα positive cells was observed in the lamina epithelialis and lining epithelium of endometrial glands during follicular phase as compared to the luteal phases of estrous cycle (P < 0.05). Higher number of PR positive cells was observed in lamina epithelialis as compared to stromal cells and smooth muscle cells in myometrium (P < 0.05). Higher percentage of PR positive cells was observed in the lamina epithelialis during follicular phase as compared to the luteal phases of estrous cycle (P < 0.05). There was no significant difference in the immuno reactivity in stromal cells, lining epithelium of endometrial glands and smooth muscle cells of myometrium during the phases of estrous cycle. The study concluded that ERα and PR expressions were higher during follicular phase as compared to the luteal phase
ABSTRACT
The study was conducted on the testes of 18 buffalo foetii to reveal histogenesis and differentiation of different cells of testicular parenchyma. At 8.0 cm CVR (65 days) the seminiferous tubules were present at gonadal periphery and a network of polygonal mesenchymal cells was seen in the centre of testis. These tubules were surrounded by a distinct basement membrane and a single layer of peritubular cells at 10 cm CVR (74 days), which became double layered at 88.0 cm CVR (272 days). The testicular parenchyma at 12.0 cm CVR had two zones; outer zone having longitudinal tubules and inner zone having rounded tubules. But a reverse pattern of their arrangement was observed at 14.0 cm CVR (92 days). The pre-Sertoli cells were first observed in buffalo foetii of 8.0 cm CVR (65 days) in the periphery of seminiferous tubular epithelium whereas the gonocytes were demonstrable in the centre of tubules at 10.6 cm CVR (76 days). The fetal Leydig cells were also reported at 8.0 cm CVR (65 days) but at 14.0 cm CVR (92 days), the interstitium had considerably expanded due to the differentiation of mesenchymal cells into the Leydig cells.
El estudio fue realizado en los testículos de 18 fetos de búfalos, para revelar la histogénesis y diferenciación de las diferentes células de parénquima testicular. A los 8,0 cm de longitud corona-rabadilla (LCR) (65 días) los túbulos seminíferos estuvieron presentes en la periferia de la gónada y una red poligonal de células mesenquimales se observó en el centro del testículo. Estos túbulos estaban rodeados por una membrana basal y una sola capa de células peritubular a los 10 cm LCR (74 días), la cual se convirtió en una doble capa a los 88,0 cm LCR (272 días). El parénquima testicular a 12,0 cm LCR tenía dos zonas, zona exterior con túbulos longitudinales y zona interior con los túbulos redondeados transversalmente. Sin embargo, un patrón inverso en su disposición se observó a los 14,0 cm LCR (92 días). Las células pre-Sertoli se observaron primero en fetos de búfalos de 8,0 cm LCR (65 días) en la periferia del epitelio seminífero tubular, mientras que los gonocitos fueron visibles en el centro de los túbulos a 10,6 cm LCR (76 días). Las células de Leydig fetales también se observaron a los 8,0 cm LCR (65 días), pero a los 14,0 cm LCR (92 días), el intersticio tuvo una considerable expansión debido a la diferenciación de células mesenquimales en células de Leydig.